How Do You Prepare Samples For Electrophoresis?

Electrophoresis has a lot of use in biotechnology. It is used as a separation method where molecules and chemicals are separated on the basis of electric charge. In other words, molecules move by virtue of attraction to an electric charge. They move through a permeable gel that permits smaller molecules to move quicker than larger molecules.  .  Applications of electrophoresis pertain to analysis of DNA molecules for identification purpose in forensics.  

             To begin with, place the gel on its casting tray into the gel box and add TBE buffer (Tris/Borate/EDTA, a buffer solution that comprises of a mixture of Tris base, boric acid, EDTA, and water.) to the gel chamber. Keep adding till there is 3 mm buffer on top of the gel. Put the numbered dye trials into the wells situated in the centre of the Agarose Gel. Load 10 ml per well. Link the electrophoresis unit to power supply (red to red, black to black), and pay attention not to knock off your gel too much. Plug the power source into an outlet. Now turn on the power supply and keep the voltage anywhere between 100-125 V. The dyes will start to move either the negative or positive pole depending on their charge.  Let the samples move for ten minutes and then turn off the power supply.